Reference Gene U2 Enables Direct Comparison between Relative Gene Expression Levels of Vascular Smooth Muscle Cells in Tissue and Culture Using Real-Time Quantitative PCR
In nearly every lab, real-time quantitative polymerase chain reaction (qPCR) is used to quantify gene expression.However, a comparison of different samples requires the 5 97 color touch careful selection of suitable reference genes (RGs), sometimes referred to as housekeeping genes.In the case of vascular smooth muscle cells (vSMCs), it is importan